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Taq Polymerase (EC 2.7.7.7): With Particular Emphasis on Its Use in PCR Protocols

Axel Landgraf, Heiner Wolfes

Open publisher page 3 citations

Abstract

Taq polymerase (EC 2.7.7.7) is a thermostable DNA-dependent DNA polymerase that was first isolated in 1976 from Thermus aquaticus strain YT-1 (ATTC # 25 104)(). It catalyzes the template-directed polymerization of dNTPs at high temperatures. Open image in new window When Taq polymerase was isolated in 1976, nobody could have known the impact the application of Taq polymerase to the polymerase chain reaction (PCR) would have on molecular biology (,). In an issue of Science in 1989, the enzyme was dubbed molecule of the year ().

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What this paper is about

Taq polymerase (EC 2.7.7.7) is a thermostable DNA-dependent DNA polymerase that was first isolated in 1976 from Thermus aquaticus strain YT-1 (ATTC # 25 104)(). It catalyzes the template-directed polymerization of dNTPs at high temperatures. Open image in new window When Taq polymerase was isolated in 1976, nobody could have known the impact the application of Taq polymerase to the polymerase chain reaction (PCR) would have on molecular biology (,). In an issue of Science in 1989, the enzyme was dubbed molecule of the year ().

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OpenAlex reports 3 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Taq polymerase (EC 2.7.7.7) is a thermostable DNA-dependent DNA polymerase that was first isolated in 1976 from Thermus aquaticus strain YT-1 (ATTC # 25 104)(). It catalyzes the template-directed polymerization of dNTPs at high temperatures. Open image in new window When Taq polymerase was isolated in 1976, nobody could have known the impact the application of Taq polymerase to the polymerase chain reaction (PCR) would have on molecular biology (,). In an issue of Science in 1989, the enzyme was dubbed molecule of the year ().

Key concepts: Thermus aquaticus, Taq polymerase, Hot start PCR, Polymerase chain reaction optimization, Polymerase, DNA polymerase, Molecular biology, Polymerase chain reaction

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