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2010•Chih Wu Sheng Li Hsueh T'ung HsunRequires access

cDNA library construction of Vitis amurensis berry skins and a preliminary analysis of the expressed sequence tags (ESTs).

Feng Li, Chengjun Yang, Bo Li, Wang Jun, Haifeng Liu

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Abstract

A cDNA library from the amur grape(Vitis amurensis Rupr.) was constructed by using Creator~(TM) SMART~(TM) cDNA Library Construction protocol.The library capacity was 1.001×10~6 with a recombinant rate of 94.11%and the inserted cDNA fragments ranged from 0.25 to 1.9 kb.935 available ESTs through random sequencing were obtained,and 636 unigenes which including 98 contigs and 201 singlets were generated after clustering.This study is propitious to gene cloning and functional genomic research of amur grape.

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What this paper is about

A cDNA library from the amur grape(Vitis amurensis Rupr.) was constructed by using Creator~(TM) SMART~(TM) cDNA Library Construction protocol.The library capacity was 1.001×10~6 with a recombinant rate of 94.11%and the inserted cDNA fragments ranged from 0.25 to 1.9 kb.935 available ESTs through random sequencing were obtained,and 636 unigenes which including 98 contigs and 201 singlets were generated after clustering.This study is propitious to gene cloning and functional genomic research of amur grape.

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Available abstract

A cDNA library from the amur grape(Vitis amurensis Rupr.) was constructed by using Creator~(TM) SMART~(TM) cDNA Library Construction protocol.The library capacity was 1.001×10~6 with a recombinant rate of 94.11%and the inserted cDNA fragments ranged from 0.25 to 1.9 kb.935 available ESTs through random sequencing were obtained,and 636 unigenes which including 98 contigs and 201 singlets were generated after clustering.This study is propitious to gene cloning and functional genomic research of amur grape.

Key concepts: Contig, cDNA library, Complementary DNA, Expressed sequence tag, Biology, Cloning (programming), Genomic library, Genetics

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cDNA library construction of Vitis amurensis berry skins and a preliminary analysis of the expressed sequence tags (ESTs). — Research Paper | ScholarLens