2009•Anhui Nongye Daxue xuebaoRequires access
Construction of cDNA library of Camellia sinensis cv. Ziyang 1 and primary analysis of expressed sequence tags (ESTs).
Donghua Li, Yao LiJuan, Youben Yu, Changjun Jiang, T. Zhou, Wu Li
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Abstract
Camellia sinensis cv.Ziyang 1 is a specific tea germplasm without flower and fruit during the whole year.A tender shoots cDNA library from the Camellia sinensis cv.Ziyang 1 was constructed by using Creator SMARTTM cDNA Library Construction Protocol.The library capacity was 1.0×106 with a recombinant rate of 93% and the inserted cDNA fragments ranged from 0.5 to 2.0 kb.594 available ESTs through random sequencing were obtained,and 299 non-repetitive sequences including 98 contigs and 201 singletons were generated after clustering.227 ESTs with known or putative function and 46 ESTs with unknown protein were identified by BlastX match of NCBI databases.The rest 26 ESTs were novel gene sequences.This study lay a foundation for gene cloning and functional genomic research of tea plant.