2010•Zhongguo Yike Daxue xuebaoRequires access
The effects of SH3GL2 gene on proliferation, apoptosis and chemotherapeutic sensibility in laryngeal cancer Hep2 cell.
Chao Shang, Yan Guo, Li Wei, FU Wei-neng, Kailai Sun
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Abstract
Objective To investigate the effects of SH3GL2 on apoptosis,proliferation and sensitivity to cis-diamminedichloroplatinum (DDP)in Hep2 cell. Methods After pcDNA3.1-SH3GL2 was constructed and transfected into Hep2 cell,the proliferation of the transfected cells was determined by methyl thiazolyl tetrazolium (MTT)and the apoptosis by flow cytometry with the treatment of DDP. Results The expression of SH3GL2 was increased after Hep2 cells were transfected with pcDNA3.1-SH3GL2,which was successfully constructed. Compared with control,the proliferation of transfectant cells was inhibited significantly (P 0.05),and significant increase of their apoptosis was observed(P 0.05). Compared with DDP-treated group,the proliferation was more significantly inhibited(P 0.05),and more significant increase of apoptosis was found in pcDNA3.1-SH3GL2-transfected cells (P 0.05). Conclusion SH3GL2 gene can inhibit proliferation and increase apoptosis of Hep2 cell,and can increase the sensitivity to DDP.