The effect of butylphthalide on neuron apoptosis,expression of NGF and BDNF in ischemia stroke tissue of rat cerebrum

Objective To study the effect of butylphthalide on neurons apoptosis,expression of NGF and BDNF in medullary ischemia of rat cerebrum. Methods The Wistar rats were divided randomly into three groups,sham-operated group,butylphthalide-creating and medullary ischemia group. The model of medullary ischemia in rats were ligated the right common carotid artery and blocked the bilateral vertebral by electrocoagulation. The rats in the butylphthalide treatment group and ischemia control group respectively received butylphthalide and normal saline by intraperitoneal injections. Nissl staining and Tunel method were used to show the neurons and apoptotic cells respectively. The immunohistochemistry was used to evaluate effects of Butylphthalide on NGF and BDNF expression. We observed brain neuron apoptosis density through the optical microscope to observe brain neuron apoptosis density,grayscale values of NGF and BDNF plays,using the grey value were analyzed. Results Treatment group of neurons apoptosis,model NGF and BDNF grayscale values were lower than control group ischemia. Conclusion Model NGF,BDNF level are closely associated with neurons density. Comparing to control group,butylphthalide can significantly up-regulate the expressions of NGF and BDNF in genetic transcription level,and protect from the medullary ischemia injury.