ScholarLens Back to search
2010•Xiandai shengwu yixue jinzhanRequires access

Effect of free fatty acids on proliferation of human periodontal ligament fibroblasts

Mei Yang, Ning Wu, Wang Wan-chun

Open publisher page 0 citations

Abstract

Objective: To investigate the effect of free fatty acids (FFAs) on proliferation of human periodontal ligament fibroblasts (hPDLFs) in vitro and to study the roles of free fatty acids in the mechanism between the metabolic syndrome (MetS) and periodontitis. Methods: HPDLFs were cultured in virto which played vital role in the periodontium tissue repaired. HPDLFs were deal with various concentrations of FFAs for 24h-72h and fibroblast proliferation was measured by colorimetric3[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Results: FFAs inhibited the proliferation of hPDLFs in a dose-and time-dependent manner with MTT assay (P0.01 vs. control respectively), the inhibition rate is the best significantly after cultured 72h (P0.01). Conclusion: FFAs inhibits the proliferation ofhPDLFs in vitro and which lead to the delayed repare ofthe periodontal tissue in obeses patients.

About this research paper

What this paper is about

Objective: To investigate the effect of free fatty acids (FFAs) on proliferation of human periodontal ligament fibroblasts (hPDLFs) in vitro and to study the roles of free fatty acids in the mechanism between the metabolic syndrome (MetS) and periodontitis. Methods: HPDLFs were cultured in virto which played vital role in the periodontium tissue repaired. HPDLFs were deal with various concentrations of FFAs for 24h-72h and fibroblast proliferation was measured by colorimetric3[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Results: FFAs inhibited the proliferation of hPDLFs in a dose-and time-dependent manner with MTT assay (P0.01 vs. control respectively), the inhibition rate is the best significantly after cultured 72h (P0.01). Conclusion: FFAs inhibits the proliferation ofhPDLFs in vitro and which lead to the delayed repare ofthe periodontal tissue in obeses patients.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective: To investigate the effect of free fatty acids (FFAs) on proliferation of human periodontal ligament fibroblasts (hPDLFs) in vitro and to study the roles of free fatty acids in the mechanism between the metabolic syndrome (MetS) and periodontitis. Methods: HPDLFs were cultured in virto which played vital role in the periodontium tissue repaired. HPDLFs were deal with various concentrations of FFAs for 24h-72h and fibroblast proliferation was measured by colorimetric3[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Results: FFAs inhibited the proliferation of hPDLFs in a dose-and time-dependent manner with MTT assay (P0.01 vs. control respectively), the inhibition rate is the best significantly after cultured 72h (P0.01). Conclusion: FFAs inhibits the proliferation ofhPDLFs in vitro and which lead to the delayed repare ofthe periodontal tissue in obeses patients.

Key concepts: Periodontal fiber, Periodontium, In vitro, Periodontitis, Fibroblast, MTT assay, Chemistry, Pharmacology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effect of free fatty acids on proliferation of human periodontal ligament fibroblasts — Research Paper | ScholarLens