Propagation of Salivary Gland Virus of the Mouse in Tissue Cultures.

Cytologic changes including large intranuclear inclusions were produced in cultures of mouse tissue inoculated with salivary gland material from mice infected with the salivary gland virus. They also occurred in 2 serial subcultures. The large intranuclear inclusions resembled those occurring in cells of the mouse salivary gland. Further evidence that the mouse salivary gland virus has been propagated in serial passages in cultures derived from mouse embryonic tissue is as follows. After 3 serial passages of the infective agent in the cultures the salivary gland virus disease, as characterized by specific intranuclear inclusions, has been reproduced in the salivary glands of mice by intraperitoneal inoculation of the supernatant fluids from the cultures. Also the infective titers of supernatant fluids withdrawn from cultures at intervals have been determined by titrations in mice. The results of the titrations, together with the calculated dilutions of the original inocula effected during the course of the experiments, demonstrated significant increase in the virus in 3 serial passages in tissue culture.