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[Advantages of spectrophotometric reading of ELISA plates for determination of the HBs/anti-HB system].

Gloria Taliani, Giovanna Palombo, M G Tonelli, Elisabetta Franco

Abstract

It has been tested some diluted HBsAg and anti-HBs positive sera by modified ELISA test. We have found a correlation between HBsAg titer and spectrophotometric reading value and we have shown it in a curve. On the other hand, by neutralization of different dilutions of a RIA anti-HBs positive serum with different dilutions of a HBsAg positive serum we have carried out a checkboard and we have found that the best titer for neutralizing HBsAg is 30 ng/ml. This HBsAg has been called "standard". Using HBsAg "standard" we have tested 50 sera of hemophiliacs and we have found 1 HBsAg positive (2%) and 38 anti-HBs positive patients (76%).

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Available abstract

Key concepts: Serial dilution, HBsAg, Titer, Virology, Standard curve, Medicine, Antibody, Chemistry