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Biosynthesis of C14-Labeled Benzylpenicillin

Oldrich K. Sebek

Open publisher page 15 citations

Abstract

The biosynthesis of the C14 labeled benzylpenicillin was achieved by preparing phenylacetic acid-l-C14. and incorporating the latter into the side-chain of benzyl-penicillin. The antibiotic was successfully separated from the nonreacted labeled phenylacetic acid by column chromatography. The data obtained by radioautography and chemical degradation of the purified antibiotic indicated that the radioactivity resided in the side-chain of the benzylpenicillin molecule.

About this research paper

What this paper is about

The biosynthesis of the C14 labeled benzylpenicillin was achieved by preparing phenylacetic acid-l-C14. and incorporating the latter into the side-chain of benzyl-penicillin. The antibiotic was successfully separated from the nonreacted labeled phenylacetic acid by column chromatography. The data obtained by radioautography and chemical degradation of the purified antibiotic indicated that the radioactivity resided in the side-chain of the benzylpenicillin molecule.

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OpenAlex reports 15 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

The biosynthesis of the C14 labeled benzylpenicillin was achieved by preparing phenylacetic acid-l-C14. and incorporating the latter into the side-chain of benzyl-penicillin. The antibiotic was successfully separated from the nonreacted labeled phenylacetic acid by column chromatography. The data obtained by radioautography and chemical degradation of the purified antibiotic indicated that the radioactivity resided in the side-chain of the benzylpenicillin molecule.

Key concepts: Benzylpenicillin, Phenylacetic acid, Penicillin, Chemistry, Biosynthesis, Antibiotics, Chromatography, Side chain

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