Probenecid Inhibition of Bumetanide-Induced Natriuresis in the Dog

SummaryBumetanide, when infused into one renal artery at 0.05 μg/kg/min in the dog, produced a significant ipsilateral increase in the excretion of sodium and water without affecting the rate of glomerular filtration. At this dose, these responses were limited to the infused kidney. When probenecid (50 mg/kg, iv) was given prior to bumetanide to inhibit tubular secretion of this drug, the natriuretic and diurectic actions of bumetanide were blocked. The inhibition of bumetanide-induced natriuresis and diuresis by probenecid indicates that adequate luminal concentration is a prerequisite to the natriuretic-diuretic actions of this drug.