Evaluation of effect of Pepleomycin for tumor bearing nude mouse using 31P-NMR. HMG tumor.

The energy metabolism of human malignant melanoma from gingiva into nude mouse (HMG tumor) was studied by a 31P-NMR spectroscopy. The spectra of HMG tumor showed peaks of phosphomonoester, inorganic phosphate, phosphodiester, phosphocreatine, γ-phosphate of adenosine 5'-triphosphate, α-phosphate of adenosine 5'-triphosphate and βphosphate of adenosine 5'-triphosphate. Phosphomonoester and adenosine 5'-triphosphate were high.Phosphomonoester, adenosine 5'-triphosphate and phosphocreatine decreased and inorganic phosphate increased after i.p. injection of pepleomycin. However, there were no observable changes in the spectra of muscle.The results suggested that 31P-NMR spectroscopy was valuable to investigate the energy metabolism in tumor tissue and to evaluate the effects of chemotherapy.