Simple and Efficient Chromosome Doubling Method by Colchicine-treatment for Haploid Plantlets of Tobacco.

There was clear difference in stomatal chloroplast number between haploid and diploid tobacco plants. In this experiment, chimeral structure of colchicine-treated tobacco plants was analyzed on the basis of stomatal chloroplast number as an indicator of ploidy level. Colchicine treatment on haploid plantlets which were derived from anther culture resulted in haploid, diploid, tetraploid and aneuploid plants. However, of the colchicine-treated plants, 13 - 36 percent showed chimera, because the ploidy level was different between lower and upper leaves of the plant. Difference in ploidy level was also observed between floral organs and the leaves (Table 1).