Efficient Induction of Diploidized Plants in Anther Culture of Rice by Colchicine Pretreatment of Cold-Preserved Spikes.

Effects of colchicine pretreatment of cold-preserved spikes on the increase in ratio of diploidized fertile regenerants in anther culture of rice were studied. After 10 days of storage at 10°C, cut ends of the spikes including uninucleate pollen grains were dipped into colchicine solution at the same temperature. When the spikes were treated with 0.8% colchicine solution for 3 days, more than 50% of the regenerants, obtained in subsequent anther culture, were fertile.