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1997•Journal of Oleo ScienceOpen access

Determination of Lipase Activity by High Performance Liquid Chromatography

Makoto Fukatsu, C. T. Thorsteinson, J. K. Daun, Toshitake Tamura

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Abstract

Lipase unit is defined as that amount of enzyme needed to produce 1 μmol non-esterified fatty acid in one minute under specified conditions. Non-esterified fatty acid is traditionally measured by acid-base titration. This was done in the present study in place of High-performance liquid chromatography (HPLC) method. HPLC with a light scattering detector was conducted to measure non-esterified fatty acids to determine the activity of lipase from Candida cylindracea. HPLC result was essentially the same as that obtained by titration, the former method being more sensitive.

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Lipase unit is defined as that amount of enzyme needed to produce 1 μmol non-esterified fatty acid in one minute under specified conditions. Non-esterified fatty acid is traditionally measured by acid-base titration. This was done in the present study in place of High-performance liquid chromatography (HPLC) method. HPLC with a light scattering detector was conducted to measure non-esterified fatty acids to determine the activity of lipase from Candida cylindracea. HPLC result was essentially the same as that obtained by titration, the former method being more sensitive.

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Available abstract

Lipase unit is defined as that amount of enzyme needed to produce 1 μmol non-esterified fatty acid in one minute under specified conditions. Non-esterified fatty acid is traditionally measured by acid-base titration. This was done in the present study in place of High-performance liquid chromatography (HPLC) method. HPLC with a light scattering detector was conducted to measure non-esterified fatty acids to determine the activity of lipase from Candida cylindracea. HPLC result was essentially the same as that obtained by titration, the former method being more sensitive.

Key concepts: Lipase, High-performance liquid chromatography, Chromatography, Titration, Chemistry, Fatty acid, Triacylglycerol lipase, Enzyme

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