Production of extracellular lipase by the hexachlorocyclohexane utilizing Pseudomonas strain Ptm+

Extracellular lipase activity was detected in a culture of Pseudomonas strain Ptm + growing on hexachlorocyclohexane. Lipase activity was associated with growth of the bacterium and reached a peak at early idiophase (22 h). Following the lipase activity, bioemulsifier was produced, with maximum activity at mid-idiophase (32 h). The extracellular lipase is probably involved in the extracellular synthesis of the bioemulsifier in the culture broth.