1992•Biological Chemistry Hoppe-SeylerRequires access

Incorporation of the Enantiomers of Lipoic Acid into the Pyruvate Dehydrogenase Complex fromEscherichia coliin vivo

R Oehring, Hans Bisswanger

Abstract

The uptake of 35S-labelled enantiomers of lipoic acid into cells from Escherichia coli was studied. The R-enantiomer was taken up by a factor of two more efficiently than the S-form. Autoradiography of polyacrylamide gels of partially purified pyruvate dehydrogenase complex from these cells showed that only the R-lipoic acid was covalently incorporated as a cofactor into the dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex.

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Key concepts: Pyruvate dehydrogenase complex, Lipoic acid, Dihydrolipoyl transacetylase, Biochemistry, Pyruvate dehydrogenase phosphatase, Pyruvate decarboxylation, Cofactor, Escherichia coli