Effect of Carbon and Nitrogen Sources on Lipase Production by Isolated Lipase-Producing Soil Yeast

 Abstract—Microbial lipase can be applied in many industrial, such as detergent formulation, fat and oil degradation, pharmaceutical synthesis, cosmetics production and biodiesel preparation. Increasing in demand for lipase, increasing attention has been focused to how to produce it efficiently and economically. In this work, the effect of carbon and nitrogen sources and pH on lipase production by isolated soil yeast Candida sp. KKU-PH2-15 was investigated by shaking flask batch culture. The maximum biomass of 18.77g/L was obtained using glucose and palm oil as carbon source and inducer, yeast extract as nitrogen source at pH 6.0, 120 rpm, and 30 ◦ C for 60h of cultivation time. The highest extracellular lipase activity of lipase-producing yeast isolate was achieved at 1.2 U/mL with specific activity of 0.65U/mg. Index Terms—Lipase-producing yeaset, lipase, microbial lipase