2003•food preservation scienceOpen access

Enterococcus faecium MR006 Produces Enterocin B, Isolated from Miso

Takumi ONDA, Fujitoshi Yanagida, Masao Tsuji, Takashi Shinohara, Koki Yokotsuka

Abstract

Enterococcus faecium MR006 was isolated from Miso and found to produce a bacteriocin with strong antibacterial activity. A culture of E. faecium MR006, maintained at a constant pH of 6.0 or 7.0 at 30°C, exhibited bacteriocin activity two-fold higher than that of the same culture grown under pH-uncontrolled conditions. MR006 bacteriocin was purified to homogeneity on SDS-PAGE by hydrophobic column chromatography and gel filtration. The estimated molecular weight of the MR006 bacteriocin was approximately 5.5 kDa, based on the tricine-SDS-PAGE system. Amino acid sequencing analysis revealed that the bacteriocin was identified to enterocin B, which is a bacteriocin produced by E. faecium T136.

Open-access reader

About this research paper

What this paper is about

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Bacteriocin, Enterococcus faecium, Size-exclusion chromatography, Microbiology, Streptococcaceae, Bacteria, Chemistry, Biology