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Citrovorum Factor, Vitamin B12, and Folic Acid Activity Whole Blood of Several Species.

William H. Pfander, L.S. Dietrich, William J. Monson, A.E. Harper, Conrad Arnold Elvehjem

Open publisher page 7 citations

Abstract

(1) The whole blood of the rat, chick, ox, or sheep was found to contain little or no free L. citrovorum active material. However, demonstrable amounts of free S. faecalis activity were found. (2) The release of LCF and PGA during autolysis is dependent on the pH of the reaction mixture. Optimum values for LCF and PGA were obtained at pH 4.5 or 6.5-7.0 depending on the species involved. (3) Blood from rats on a B12 deficient diet containing iodinated casein had little LCF activity. However, blood from hyperthyroid rats receiving ample vit. B12 contained LCF levels similar to those of normal rats after fasting.

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(1) The whole blood of the rat, chick, ox, or sheep was found to contain little or no free L. citrovorum active material. However, demonstrable amounts of free S. faecalis activity were found. (2) The release of LCF and PGA during autolysis is dependent on the pH of the reaction mixture. Optimum values for LCF and PGA were obtained at pH 4.5 or 6.5-7.0 depending on the species involved. (3) Blood from rats on a B12 deficient diet containing iodinated casein had little LCF activity. However, blood from hyperthyroid rats receiving ample vit. B12 contained LCF levels similar to those of normal rats after fasting.

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Available abstract

(1) The whole blood of the rat, chick, ox, or sheep was found to contain little or no free L. citrovorum active material. However, demonstrable amounts of free S. faecalis activity were found. (2) The release of LCF and PGA during autolysis is dependent on the pH of the reaction mixture. Optimum values for LCF and PGA were obtained at pH 4.5 or 6.5-7.0 depending on the species involved. (3) Blood from rats on a B12 deficient diet containing iodinated casein had little LCF activity. However, blood from hyperthyroid rats receiving ample vit. B12 contained LCF levels similar to those of normal rats after fasting.

Key concepts: Vitamin B12, Casein, Autolysis (biology), Folic acid, Cyanocobalamin, Chemistry, Vitamin, Biochemistry

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