2010•The Research of Environmental SciencesRequires access
Screening of Aerobic Denitrifying Bacillus and Study on Denitrification Characteristics
An Jian, Song Zeng-fu, Xianle Yang, Zhihua Huang, Zhang Xiaoneng
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Abstract
Many Bacillus strains with aerobic denitrification character were isolated from the water and sediment of shrimp culture ponds.Among them,the Bacillus YX-6 could degrade nitrite nitrogen from 10(mg/L) to zero in 14 h,as well as approximately 100% of nitrite nitrogen removal rate at a DO mass concentration of 5.2-5.8(mg/L).Furthermore,in comparison with the denitrifying Bacillus selected as the positive control,the denitrification characteristics of Bacillus YX-6 were tested under different pH,temperature,nitrite concentration and salinity.The results showed that the aerobic denitrification characteristics of Bacillus YX-6 were significantly higher than those of the positive control(P0.05).According to morphological observation,physiological and biochemical properties,and 16S rDNA molecular identification,the Bacillus YX-6 was identified primarily as Bacillus coagulans.