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2010•Unpublished venueRequires access

Content Determination of Gallic Acid in Wuhuang Ointments by HPLC

Zheng Li, Zhang Xiao-qian

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Abstract

Objective To set up a method for determining the content of gallic acid in Wuhuang Gao.Methods HPLC separation was performed by gradient eluting with methanol-0.1% phosphoric acid at the flow rate of 1.0mL/min.The detection wavelength was 270 nm.Results The linear range of gallic acid was 0.189 2～1.892 μg,r =0.999 9.The average recovery rate was 99.2%,RSD=1.22%.Conclusion The method is highly effective,quick and sensitive,and suitable for quality control of Wuhuang Ointments.

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What this paper is about

Objective To set up a method for determining the content of gallic acid in Wuhuang Gao.Methods HPLC separation was performed by gradient eluting with methanol-0.1% phosphoric acid at the flow rate of 1.0mL/min.The detection wavelength was 270 nm.Results The linear range of gallic acid was 0.189 2～1.892 μg,r =0.999 9.The average recovery rate was 99.2%,RSD=1.22%.Conclusion The method is highly effective,quick and sensitive,and suitable for quality control of Wuhuang Ointments.

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Available abstract

Objective To set up a method for determining the content of gallic acid in Wuhuang Gao.Methods HPLC separation was performed by gradient eluting with methanol-0.1% phosphoric acid at the flow rate of 1.0mL/min.The detection wavelength was 270 nm.Results The linear range of gallic acid was 0.189 2～1.892 μg,r =0.999 9.The average recovery rate was 99.2%,RSD=1.22%.Conclusion The method is highly effective,quick and sensitive,and suitable for quality control of Wuhuang Ointments.

Key concepts: Gallic acid, Phosphoric acid, Content determination, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Recovery rate

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