Capillary Electrophoresis Determination of the Enantiomeric Purity of Four Compounds Obtained from Asymmetry Synthesis

The enantioseparation of four compounds, 3-(diethylamino)-1-phenylpropan-1-ol(a), 1-phenyl-3-(piperidin-1-yl)propan-1-ol(b), 3-morpholino-1-p-tolypropan-1-ol(c) and 1-(4-chlorophenyl)-3-morpholinopropan-1-ol(d), obtained from asymmetry synthesis, was studied by capillary electrophoresis using α-cyclodextrin, β-cyclodextrin and β-cyclodextrin polymer as chiral selectors. With 20 kV as the separation voltage, and 30 g/L β-cyclodextrin polymer in 80 mmol/L Tris(pH=3.2) as background electrolyte solution, four chiral compounds were enantioseparated in 40 min. The enantiomeric purity of these four chiral compounds was determined according to the ratio of peak area with RSD no more than 1.5%.