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2004•International Journal of Agriculture and BiologyRequires access

Micropropagation of sugarcane through apical bud and axillary bud

Kumkum Cheema, Makhdoom Hussain

Open publisher page 32 citations

Abstract

Micropropagation study of sugarcane (Saccharum officinarum) related that for multiplication of desirable clone apical or axillary bud might be used to get easy and faster shoot initiation. By keeping the cultured tube (media portion) under dark, phenolic exudation could be controlled. Genotypic response for shoot initiation, shoot length, shoot multiplication cued rooting was different to different media formulation. Maximum survival of explant of SPF 234 and CP 77/400 was observed on M3. M2 was better media than other for CPF 237 and SPF 213. CP43/33 showed survival on wider range of cytokinins (M1 to M4). HSF240 performed well on M5. SPF 213 produced maximum root length (3.20 cm) after 30 days; whereas, minimum root length of (0.43 cm) was observed in varieties CP77/400, with 10 cm shoot length.

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What this paper is about

Micropropagation study of sugarcane (Saccharum officinarum) related that for multiplication of desirable clone apical or axillary bud might be used to get easy and faster shoot initiation. By keeping the cultured tube (media portion) under dark, phenolic exudation could be controlled. Genotypic response for shoot initiation, shoot length, shoot multiplication cued rooting was different to different media formulation. Maximum survival of explant of SPF 234 and CP 77/400 was observed on M3. M2 was better media than other for CPF 237 and SPF 213. CP43/33 showed survival on wider range of cytokinins (M1 to M4). HSF240 performed well on M5. SPF 213 produced maximum root length (3.20 cm) after 30 days; whereas, minimum root length of (0.43 cm) was observed in varieties CP77/400, with 10 cm shoot length.

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Available abstract

Micropropagation study of sugarcane (Saccharum officinarum) related that for multiplication of desirable clone apical or axillary bud might be used to get easy and faster shoot initiation. By keeping the cultured tube (media portion) under dark, phenolic exudation could be controlled. Genotypic response for shoot initiation, shoot length, shoot multiplication cued rooting was different to different media formulation. Maximum survival of explant of SPF 234 and CP 77/400 was observed on M3. M2 was better media than other for CPF 237 and SPF 213. CP43/33 showed survival on wider range of cytokinins (M1 to M4). HSF240 performed well on M5. SPF 213 produced maximum root length (3.20 cm) after 30 days; whereas, minimum root length of (0.43 cm) was observed in varieties CP77/400, with 10 cm shoot length.

Key concepts: Shoot, Axillary bud, Micropropagation, Biology, Saccharum officinarum, Explant culture, Botany, Apical dominance

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