2009•Unpublished venueRequires access
Rapid In vitro Micro propagation of Sugarcane (Saccharum officinarum L. cv- Nayana) Through Callus Culture
B H Kambaska, S Santilata
Open publisher page 50 citations
Abstract
Standardization of protocol for induction of callus and regeneration of plantlets was established through in vitro culture using young meristem of Sugarcane (Saccharum officinarum L. cv- Nayana) as an explant. The multiple shoot regeneration at various frequencies was observed by using different concentration and combination of growth regulators. The highest percentage of callus induction was observed in MS medium supplemented with 2.5 mg/l, 2-4 D. The best response in terms of multiple shoot induction was observed on MS medium with BAP 2.0 mg/l + NAA 0.5 mg/l. When in vitro shoot lets were inoculated on to the half-strength MS basal media supplemented with 3.0 mg/l NAA, rooting was more profuse. Rooted shoots were transplanted in the green house for hardening and their survival rate was 90% in the field condition. (Nature and Science. 2009;7(4):1-10). (ISSN: 1545-0740).