Optimization of Process Parameters for the Production of Tannase and Gallic Acid by Enterobacter Cloacae MTCC 9125.

Tannase and gallic acid production by Enterobacter cloacae MTCC 9125 was optimized. The organism produced maximum enzyme and gallic acid at initial medium pH 4.5 and cultivation temperature of 37 0 C after 48 h of incubation period. 1% of 24 h old inoculumn was found to be optimum for tannase production. However, 48 h old inoculumn showed maximum gallic acid accumulation. Supplement of carbohydrates decreased enzyme synthesis, while fructose, sucrose and glucose increases cell mass growth.1.2% tannic acid was found to be optimum for biosynthesis of tannase and gallic acid. The organism showed maximum tannase production with sodium nitrate and KH 2 PO 4 as nitrogen source and phosphate source respectively. Ca 2+ and Mg 2+ ions were found to be stimulatory for