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2011•Asian Journal of Research in ChemistryRequires access

A New RP-HPLC Method for the Simultaneous Estimation of Rabeprazole Sodium and Itopride Hydrochloride in Formulation

D. Boopathy, Badhan A. Kumar, Devaki Magham, V. Sekar, Paramasivan Thirumalai Perumal

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Abstract

A simple, sensitive, precise, accurate, rapid and reproducible reverse phase high performance liquid chromatographic procedure is developed for simultaneous determination of Rabeprazole sodium and Itopride hydrochloride in Capsule dosage form. The mobile phase used was a combination of methanol: buffer (65:35 v/v) and the pH was found to be 4.30. The detection of the capsule dosage form was carried out at 285 nm and the flow rate employed was 1 mL/min. Linearity was obtained in the concentration range of 2.5 to 15 μg/mL of Rabeprazole sodium and 20 to 120 μg/mL of itopride hydrochloride with a correlation coefficient of 0.998 and 0.996 respectively. The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.

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What this paper is about

A simple, sensitive, precise, accurate, rapid and reproducible reverse phase high performance liquid chromatographic procedure is developed for simultaneous determination of Rabeprazole sodium and Itopride hydrochloride in Capsule dosage form. The mobile phase used was a combination of methanol: buffer (65:35 v/v) and the pH was found to be 4.30. The detection of the capsule dosage form was carried out at 285 nm and the flow rate employed was 1 mL/min. Linearity was obtained in the concentration range of 2.5 to 15 μg/mL of Rabeprazole sodium and 20 to 120 μg/mL of itopride hydrochloride with a correlation coefficient of 0.998 and 0.996 respectively. The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.

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Available abstract

A simple, sensitive, precise, accurate, rapid and reproducible reverse phase high performance liquid chromatographic procedure is developed for simultaneous determination of Rabeprazole sodium and Itopride hydrochloride in Capsule dosage form. The mobile phase used was a combination of methanol: buffer (65:35 v/v) and the pH was found to be 4.30. The detection of the capsule dosage form was carried out at 285 nm and the flow rate employed was 1 mL/min. Linearity was obtained in the concentration range of 2.5 to 15 μg/mL of Rabeprazole sodium and 20 to 120 μg/mL of itopride hydrochloride with a correlation coefficient of 0.998 and 0.996 respectively. The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.

Key concepts: Chromatography, Chemistry, Rabeprazole, Capsule, Correlation coefficient, Methanol, Sodium, High-performance liquid chromatography

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A New RP-HPLC Method for the Simultaneous Estimation of Rabeprazole Sodium and Itopride Hydrochloride in Formulation — Research Paper | ScholarLens