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2014•Asian Journal of Research in ChemistryRequires access

Development and Validation of UV Spectrophotometric Method of Amlodipine Besylste in Bulk and Pharmaceutical Formulation

G. Raveendra Babu, J. Praveen Kumar, P. Sri Lakshmi Surekha, T. Kala Praveen, Prasada Rao

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Abstract

A simple, accurate, cost effective and reproducible spectrophotometric method has been developed for the estimation of Amlodipine besylate in bulk and pharmaceutical dosage form. UV spectrophotometric method, which is based on measurement of absorption at maximum wavelength 365nm. The percentage recovery of Amlodipine besylate ranged from (99.80 ± 1.09) in pharmaceutical dosage form. The developed method was validated with respect to linearity, accuracy (recovery), precision and specificity. Beers law was obeyed in the concentration range of 5–25μg/ml having line equation y = Y=0.0163C - 0.00120 with correlation coefficient of 0.9999. Results of the analysis were validated statistically and by recovery study.

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What this paper is about

A simple, accurate, cost effective and reproducible spectrophotometric method has been developed for the estimation of Amlodipine besylate in bulk and pharmaceutical dosage form. UV spectrophotometric method, which is based on measurement of absorption at maximum wavelength 365nm. The percentage recovery of Amlodipine besylate ranged from (99.80 ± 1.09) in pharmaceutical dosage form. The developed method was validated with respect to linearity, accuracy (recovery), precision and specificity. Beers law was obeyed in the concentration range of 5–25μg/ml having line equation y = Y=0.0163C - 0.00120 with correlation coefficient of 0.9999. Results of the analysis were validated statistically and by recovery study.

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Available abstract

A simple, accurate, cost effective and reproducible spectrophotometric method has been developed for the estimation of Amlodipine besylate in bulk and pharmaceutical dosage form. UV spectrophotometric method, which is based on measurement of absorption at maximum wavelength 365nm. The percentage recovery of Amlodipine besylate ranged from (99.80 ± 1.09) in pharmaceutical dosage form. The developed method was validated with respect to linearity, accuracy (recovery), precision and specificity. Beers law was obeyed in the concentration range of 5–25μg/ml having line equation y = Y=0.0163C - 0.00120 with correlation coefficient of 0.9999. Results of the analysis were validated statistically and by recovery study.

Key concepts: Amlodipine, Dosage form, Correlation coefficient, Beer–Lambert law, Chromatography, Linearity, Pharmaceutical formulation, Chemistry

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Development and Validation of UV Spectrophotometric Method of Amlodipine Besylste in Bulk and Pharmaceutical Formulation — Research Paper | ScholarLens