Composition analysis of HMW-GS in Australian wheat cultivars.
FU Xiao-yi, Jia Dan, Mengjun Li, He Mingqi, Shi Zhanliang, Cai Xin, Jinkao Guo
Abstract
FU Xiao-yi, Jia Dan, Mengjun Li, He Mingqi, Shi Zhanliang, Cai Xin, Jinkao Guo
Abstract
[Objective] The aim was to provide information for the breeding of wheat in China.[Method] 64 wheat cultivars from Australia were analyzed by SDS-PAGE to determine their high molecular weight(HMW) glutenin subunit combinations.Then,the cultivars had good HMW-GS were screened.[Result] A total of nine alleles were identified at Glu-1.Glu-A1 encoded two subunits,1 and null subunit,and 1 was the major type,the frequency was 59.37%;Glu-B1 encoded five subunits,7,7+8,7+9,14+15,17+ 18,and 7+8 was the major type,the frequency was 56.20%;Glu-D1 encoded two subunits,2+12,5+10,and 2+12 was the major type,the frequency was 70.30%.Furthermore,12 subunit combinations were detected,and the composition of 1,2+12,7+8 was the major type.The quality scores of these cultivars ranged from 4 to 10,with an average of 7.4.[Conclusion] The quality of these varieties was good.
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[Objective] The aim was to provide information for the breeding of wheat in China.[Method] 64 wheat cultivars from Australia were analyzed by SDS-PAGE to determine their high molecular weight(HMW) glutenin subunit combinations.Then,the cultivars had good HMW-GS were screened.[Result] A total of nine alleles were identified at Glu-1.Glu-A1 encoded two subunits,1 and null subunit,and 1 was the major type,the frequency was 59.37%;Glu-B1 encoded five subunits,7,7+8,7+9,14+15,17+ 18,and 7+8 was the major type,the frequency was 56.20%;Glu-D1 encoded two subunits,2+12,5+10,and 2+12 was the major type,the frequency was 70.30%.Furthermore,12 subunit combinations were detected,and the composition of 1,2+12,7+8 was the major type.The quality scores of these cultivars ranged from 4 to 10,with an average of 7.4.[Conclusion] The quality of these varieties was good.
Key concepts: Glutenin, Cultivar, Protein subunit, Composition (language), Biology, Common wheat, Allele, Food science