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A Most Probable Number Method for the Enumeration of Legionella Bacteria in Water

Niels Grabow, R. Kfir, W. O. K. Grabow

Abstract

A new quantitative method for the enumeration of Legionella bacteria in water is described. Appropriate tenfold serial dilutions of water samples concentrated by membrane filtration are plated in triplicate on buffered charcoal yeast extract agar. After incubation for 3 days representative smears from individual plates are tested for the presence of Legionella by direct fluorescent antibody staining. The number of positive plates in each dilution is used to calculate the Legionella count by means of conventional most probable number statistics. In comparative tests on a variety of water samples this method yielded significantly higher counts than previously used procedures.

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Key concepts: Enumeration, Serial dilution, Legionella, Most probable number, Agar, Bacteria, Microbiology, Chromatography