2014•Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)Open access
Immobilization of whole cells of Yarrowia lipolytica for citric acid production
Antónia Gonçalves, Adelaide Braga, Patrícia Ferreira, Isabel Belo
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Abstract
Yarrowia lipolytica is one of the most extensively studied ‘‘non-conventional’’ yeast species, highlighted by their participation in bioremediation processes and production of lipases, γ-decalactone and citric acid. The use of immobilized cells presents several advantages over free cells in bioprocesses. In this work, Y. lipolytica entrapment in calcium alginate was applied for citric acid production from glycerol. The influence of sodium alginate and calcium chloride concentrations on the immobilization efficiency was investigated, being selected 4 % of sodium alginate and 0.5 M of calcium chloride, the best conditions. Afterwards, citric acid production was assessed at several spheres diameter and cells concentration, but no significant differences of final citric acid concentration were observed.
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