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Improved Survival Under Vibrio Challenge and Enhanced Immunogene Transcription of the Pacific White Shrimp, Litopenaeus vannamei, which were Oral Administrated with Brown Algae Extract, Endarachne binghamiae, Via Enriched Artemia

Saou-Lien Wong, Chun‐Hung Liu, Winton Cheng, Shinn‐Pyng Yeh

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Abstract

White shrimp, Litopenaeus vannamei (5±0.6 g), which received the extract from the brown alga, Endarachne binghamiae (EB), at 20 μg shrimp^(-1) showed a significantly increased survival rate after challenge with Vibrio alginolyticus. Lipopolysaccharide- and β-1, 3-glucan-binding protein (LGBP), prophenoloxidase (proPO), peroxinectin (PE), glutathione peroxidase (GPX), lysozyme (LYS), and serine proteinase (SP) gene expression were used as immune indicators to evaluate the state of immunity of white shrimp postlarvae (PL10) after having been individually fed Artemia nauplii which had been exposed to 0, 5, and 10 gl^(-1) of the extract of EB for 4 h. Results showed that the expressions of LGBP, PE, GPX, and LYS were significantly elevated in shrimp postlarvae fed the extract of EB-enriched Artemia nauplii after 144 h. It was concluded that administration of the extract of EB induces immune modulation, enhances the immune ability of L. vannamei, and increase its resistance to V. alginolyticus infection.

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What this paper is about

White shrimp, Litopenaeus vannamei (5±0.6 g), which received the extract from the brown alga, Endarachne binghamiae (EB), at 20 μg shrimp^(-1) showed a significantly increased survival rate after challenge with Vibrio alginolyticus. Lipopolysaccharide- and β-1, 3-glucan-binding protein (LGBP), prophenoloxidase (proPO), peroxinectin (PE), glutathione peroxidase (GPX), lysozyme (LYS), and serine proteinase (SP) gene expression were used as immune indicators to evaluate the state of immunity of white shrimp postlarvae (PL10) after having been individually fed Artemia nauplii which had been exposed to 0, 5, and 10 gl^(-1) of the extract of EB for 4 h. Results showed that the expressions of LGBP, PE, GPX, and LYS were significantly elevated in shrimp postlarvae fed the extract of EB-enriched Artemia nauplii after 144 h. It was concluded that administration of the extract of EB induces immune modulation, enhances the immune ability of L. vannamei, and increase its resistance to V. alginolyticus infection.

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Available abstract

White shrimp, Litopenaeus vannamei (5±0.6 g), which received the extract from the brown alga, Endarachne binghamiae (EB), at 20 μg shrimp^(-1) showed a significantly increased survival rate after challenge with Vibrio alginolyticus. Lipopolysaccharide- and β-1, 3-glucan-binding protein (LGBP), prophenoloxidase (proPO), peroxinectin (PE), glutathione peroxidase (GPX), lysozyme (LYS), and serine proteinase (SP) gene expression were used as immune indicators to evaluate the state of immunity of white shrimp postlarvae (PL10) after having been individually fed Artemia nauplii which had been exposed to 0, 5, and 10 gl^(-1) of the extract of EB for 4 h. Results showed that the expressions of LGBP, PE, GPX, and LYS were significantly elevated in shrimp postlarvae fed the extract of EB-enriched Artemia nauplii after 144 h. It was concluded that administration of the extract of EB induces immune modulation, enhances the immune ability of L. vannamei, and increase its resistance to V. alginolyticus infection.

Key concepts: Litopenaeus, Vibrio alginolyticus, Prophenoloxidase, Shrimp, Biology, Lysozyme, Microbiology, Glutathione peroxidase

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Improved Survival Under Vibrio Challenge and Enhanced Immunogene Transcription of the Pacific White Shrimp, Litopenaeus vannamei, which were Oral Administrated with Brown Algae Extract, Endarachne binghamiae, Via Enriched Artemia — Research Paper | ScholarLens