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Plant Differentiation from Callus Tissue of Saccharum Species1

D. J. Heinz, Grace W. P. Mee

Abstract

Callus tissue was induced on parenchyma tissue of shoot apices, leaves, and inflorescences of Saccharum sp. using a basal mineral medium supplemented with coconut water (10% by volume) and 2,4‐D. Transfer of callus tissue to a non‐2,4‐D basal medium induced the differentiation of plants. In all the plants studied, morphological variants from the parental clone were observed.

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Key concepts: Callus, Biology, Saccharum, Parenchyma, Inflorescence, Botany, Tissue culture, Shoot