Extraction of Pomegranate Peel Polyphenols and Its Antimicrobia Activities
Ling Wang, Jiao Shirong, Menglin Lei, Tang Yuan-mou, Tang Peng-cheng, Xiaohong Zuo
Abstract
Ling Wang, Jiao Shirong, Menglin Lei, Tang Yuan-mou, Tang Peng-cheng, Xiaohong Zuo
Abstract
[Objective] The research aimed to optimize the extraction process of pomegranate peel polyphenols and study its antimicrobia activities.[Method] Microwave-assisted extraction(MAE) was used to extract the pomegranate peel polyphenols.And the best extract conditions was obtained by single factor experiments and orthogonal test.Then the inhibition zone diameter and minimal inhibitory concentration(MIC) of pomegranate polyphenol extracts on the Staphylococcus aureus,Proteus vulgaris and Baccillus cereus were measured.[Result] The results showed that the best extract conditions of extracting the pomegranate peel polyphenols were as follows:The item number 60~80 item,extraction power 385 W,the ratio of powder to the solution 1∶25,extraction time 120 s,extraction times 3 times respectively.Under the best extraction conditions,the yield of polyphenols from pomegranate peel was 21.4%.The most notable inhibition concentration of pomegranate peel polyphenols was the highest of 0.96 g/ ml.The inhibition effect of their counterparts in diameter were 1.15,1.10,0.95 cm and the MIC was 0.80,1.60 and 0.40 g/ml respectively.[Conclusion] The study can provide scientific basis for extracting nature medicine from pomegranate peel and finding nature bacteriostat.
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[Objective] The research aimed to optimize the extraction process of pomegranate peel polyphenols and study its antimicrobia activities.[Method] Microwave-assisted extraction(MAE) was used to extract the pomegranate peel polyphenols.And the best extract conditions was obtained by single factor experiments and orthogonal test.Then the inhibition zone diameter and minimal inhibitory concentration(MIC) of pomegranate polyphenol extracts on the Staphylococcus aureus,Proteus vulgaris and Baccillus cereus were measured.[Result] The results showed that the best extract conditions of extracting the pomegranate peel polyphenols were as follows:The item number 60~80 item,extraction power 385 W,the ratio of powder to the solution 1∶25,extraction time 120 s,extraction times 3 times respectively.Under the best extraction conditions,the yield of polyphenols from pomegranate peel was 21.4%.The most notable inhibition concentration of pomegranate peel polyphenols was the highest of 0.96 g/ ml.The inhibition effect of their counterparts in diameter were 1.15,1.10,0.95 cm and the MIC was 0.80,1.60 and 0.40 g/ml respectively.[Conclusion] The study can provide scientific basis for extracting nature medicine from pomegranate peel and finding nature bacteriostat.
Key concepts: Polyphenol, Extraction (chemistry), Chemistry, Yield (engineering), Chromatography, Food science, Traditional medicine, Antioxidant