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Comparative study on three methods of removing proteins from polysaccharide extract from Undaria pinnatifida.

Ge BingYan, Bo Yang

Open publisher page 5 citations

Abstract

Objective To remove the proteins from the polysaccharide extract from Undaria pinnatifida and select the best method of removing proteins.Methods Three different methods of removing proteins including the Sevag method,trichloroacetic acid(TCA) method and enzymic method were compared by analyzing the percentages of deproteinization and lost polysaccharide.The optimum method of removing proteins was selected.Results The enzymic method had a lower percentage of lost polysaccharide,which was 10.22 %,but a high percentage of deproteinization,which was 61.10 %.For TCA,the percentage of deproteinization was 57.37 %,the percentage of lost polysaccharide was 12.13 %.The percentages of deproteinization and lost polysaccharide of Sevag method were 54.12 % and 14.21 %,respectively.Conclusion The enzymic method can be the best method of removing proteins from the polysaccharide extract from Undaria pinnatifida.

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What this paper is about

Objective To remove the proteins from the polysaccharide extract from Undaria pinnatifida and select the best method of removing proteins.Methods Three different methods of removing proteins including the Sevag method,trichloroacetic acid(TCA) method and enzymic method were compared by analyzing the percentages of deproteinization and lost polysaccharide.The optimum method of removing proteins was selected.Results The enzymic method had a lower percentage of lost polysaccharide,which was 10.22 %,but a high percentage of deproteinization,which was 61.10 %.For TCA,the percentage of deproteinization was 57.37 %,the percentage of lost polysaccharide was 12.13 %.The percentages of deproteinization and lost polysaccharide of Sevag method were 54.12 % and 14.21 %,respectively.Conclusion The enzymic method can be the best method of removing proteins from the polysaccharide extract from Undaria pinnatifida.

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Available abstract

Objective To remove the proteins from the polysaccharide extract from Undaria pinnatifida and select the best method of removing proteins.Methods Three different methods of removing proteins including the Sevag method,trichloroacetic acid(TCA) method and enzymic method were compared by analyzing the percentages of deproteinization and lost polysaccharide.The optimum method of removing proteins was selected.Results The enzymic method had a lower percentage of lost polysaccharide,which was 10.22 %,but a high percentage of deproteinization,which was 61.10 %.For TCA,the percentage of deproteinization was 57.37 %,the percentage of lost polysaccharide was 12.13 %.The percentages of deproteinization and lost polysaccharide of Sevag method were 54.12 % and 14.21 %,respectively.Conclusion The enzymic method can be the best method of removing proteins from the polysaccharide extract from Undaria pinnatifida.

Key concepts: Polysaccharide, Trichloroacetic acid, Chemistry, Chromatography, Biochemistry

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