2010•Unpublished venueRequires access

Inhibitory mechanism of oridonin on G2/M phase arrest in SGC-7901 cell growth.

JI Yu-bin, Hong Bao, Gao Shi-yong

Abstract

Objective To investigate the effect of oridonin on G2/M phase arrest of human stomach cancer SGC-7901 cell growth in vitro and its molecular mechanism.Methods The inhibition of oridonin on SGC-7901 cells was detected by MTT assay.Effect of oridonin on the phase distribution of SGC-7901 cell cycle was observed by flow cytometry(FCM).Western blotting was used to analyze the expression of Cdk1 and CyclinB1.Results IC50 of oridonin on SGC-7901 cells was 15.64 μmol/L.The effect of oridonin on SGC-7901 cells presented the increasing percentages of cells in G2/M phase as the concentration of oridonin increased.With the increase of oridonin dose,the expresstion of Cdk1 and CyclinB1 proteins was remarkably decreased.Conclusion Oridonin can down-regulate the expression of Cdk1 and CyclinB1,which may be the mechanism of arresting SGC-7901 cells in G2/M phase.

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Key concepts: Cyclin-dependent kinase 1, Cell cycle, Flow cytometry, Chemistry, MTT assay, IC50, Apoptosis, Cell cycle checkpoint