Comparison of drug resistant genes typing and pulsed-field gel electrophoresis typing for Acinetobacter baumannii.

[Objective] To study the epidemiology of drug resistant genes of Acinetobacter baumannii and to compare the difference between the two genotyping methods,i.e. drug resistant gene typing(DRGT)and pulsed-field gel electrophoresis(PFGE). [Methods] Twenty-seven isolates of Acinetobacter baumannii were collected in the Second Hospital of Ningbo during 2005,including both epidemic and sporadic events. Susceptibility test,PFGE,DRGT and cluster analysis were carried out to confirm the drug resistance and to analyze the genotyping,respectively.[Results] Twenty-seven strains of Acinetobacter displayed multiple antibiotic resistance and drug resistant genes. The result of DRGT based on various drug resistant genes was comparable to PFGE in Acinetobacter strains with the concordance of 88.9%,and even suggested a molecular evolution course of different drug-resistant strains.[Conclusion] Compared to PFGE,DRGT is useful to analyze molecular epidemiology of nosocomial infections,which would benefits clinical diagnosis and therapy.