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2013•Annals of HorticultureRequires access

Estimation of genetic variability among papaya (Carica papaya L.) germplasm using rapd markers

Ravindra Kumar, Mukesh Kumar, Shikha Sharma, Trivendr Singh Gangwar, Swati Singh, Vandana Tomar

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Abstract

The genetic diversity among ten papaya genotypes were investigated using five RAPD primers. DNA was extracted by CTAB method. The banding pattern was analyzed using UPGMA based on Jaccard's coefficient. All RAPD primers showed polymorphism. The dendrogram revealed two distinct clusters. Cluster I includes two genotypes which were introduced in India from other countries and having pink flesh. The PIC value for the RAPD loci ranged from 0.16 to 0.76.

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The genetic diversity among ten papaya genotypes were investigated using five RAPD primers. DNA was extracted by CTAB method. The banding pattern was analyzed using UPGMA based on Jaccard's coefficient. All RAPD primers showed polymorphism. The dendrogram revealed two distinct clusters. Cluster I includes two genotypes which were introduced in India from other countries and having pink flesh. The PIC value for the RAPD loci ranged from 0.16 to 0.76.

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Available abstract

The genetic diversity among ten papaya genotypes were investigated using five RAPD primers. DNA was extracted by CTAB method. The banding pattern was analyzed using UPGMA based on Jaccard's coefficient. All RAPD primers showed polymorphism. The dendrogram revealed two distinct clusters. Cluster I includes two genotypes which were introduced in India from other countries and having pink flesh. The PIC value for the RAPD loci ranged from 0.16 to 0.76.

Key concepts: RAPD, UPGMA, Carica, Jaccard index, Dendrogram, Germplasm, Genetic diversity, Biology

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