Method study of identification and content determination of Chuanxiong Rhizoma and Angelicae Dahuricae Radix for Duliang soft capsules and dropping pills

Objective: To develop a TLC method for identification of Chuanxiong Rhizoma and Angelicae Dahuricae Radix in Duliang soft capsules and Duliang dropping pills,and establish an HPLC method for determination of imperatorin and isoimperatorin in above 2 preparations. Methods: An expansion agent system of hexane-ethyl acetate（ 8∶ 2） was adoped for identification of Chuanxiong Rhizoma and Angelicae Dahuricae Radix. The analysis was performed on a Phenomenex Luna C18column（ 250 mm × 4. 6 mm,5 μm） with the mobile phase of acetonitrile-water（ 50∶ 50） at a flow rate of 1. 0 m L·min -1 . The detection wavelength was set at 249 nm and the column temperature was controlled at 25 ℃. Results: Main spots of Duliang soft capsules and Duliang dropping pills in TLC were clear which a good exhibited separation was observed. A good linearity was obtained with imperatorin in the range of0. 01-0. 21 μg（ r = 0. 999 5） and isoimperatorin in the range of 0. 02-0. 41 μg（ r = 0. 999 9）; the average recovery of imperatorin was 99. 9%（ n = 6） and the average recovery of isoimperatorin was 101. 7%（ n = 6） in Duliang soft capsules; the average recovery of imperatorin was 98. 7%（ n = 6） and the average recovery of isoimperatorin was96. 5%（ n = 6） in Duliang dropping pills. Conclusion: The established method is specific,accurate and reproducible,which can be used for quality control of the Duliang soft capsules and Duliang dropping pills.