Electrochemical determination of anticoccidial drug robenidine hydrochloride

The electrochemical oxidation ofrobenidine hydrochloride has been carried out in Britton-Robinson buffer at carbon paste and glassy carbon electrodes. Robenidine hydrochloride exhibits a well-defined irreversible oxidation peak over the entire pH range (2-8). Differential pulse voltammetry was used to determine robenidine hydrochloride in the pure form. The peak current varied linearly in the following ranges: 4.0 x 10 -7 - 3.2 × 10 -6 mol l -1 in the case of carbon paste electrode, and 4.0 x 10 -7 - 2.8 × 10 -6 mol l -1 with the glassy carbon electrode. In the case ofcarbon paste electrode the limits of detection and quantification were 7.33 x 10 -8 mol l -1 , and 2.44 × 10 -7 mol l -1 , respectively. For glassy carbon electrode, they were 7.93 x 10 -8 mol l -1 and 2.64 x 10 -7 mol l -1 , respectively. The percentage recoveries were found in the following ranges: 99.37-100.83 % and 99.17-101 %for carbon paste and glassy carbon electrodes, respectively. The relative standard deviations were found in the following ranges: 0.376-0.973 % and 0.657-1.28 % in the case of carbon paste and glassy carbon electrodes, respectively. Differential pulse voltammetry method was successfully applied for the determination of robenidine hydrochloride in pharmaceutical form and chicken serum.