Electrochemical determination of veterinary drug danofloxacin

The electrochemical oxidation of danofloxacin has been carried out in Britton-Robinson buffer at carbon paste and glassy carbon electrodes. Danofloxacin exhibits a well-defined irreversible oxidation peak over the entire pH range (2-10). Differential pulse voltammetry was used to determine danofloxacin in the pure form. The peak current varied linearly in the following ranges: 8.0 x 10-7 -4.4 x 10-6 mol l-1 and 4.0 x 10-7 -4.8 x 10-6 mol l-1 in the cases of carbon paste electrode and glassy carbon electrode, respectively. In the case of carbon paste electrode the limits of detection and quantification were 1.194 x 10-7 mol l-1 and 3.979 x 10-7 mol l-1, respectively. The percentage recoveries were found in the following ranges: 99.17-101.50% and 99.38-101.25% for carbon paste and glassy carbon electrodes, respectively. The relative standard deviations were found in the following ranges: 0.564-0.890 % and 0.507-0.884 % in the cases of carbon paste and glassy carbon electrodes, respectively. Differential pulse voltammetry method was successfully applied for the determination of danofloxacin in pharmaceutical form and chicken serum.