2011•Research Journal of Pharmacy and TechnologyRequires access
Simultaneous Determination of Telmisartan and Amlodipine in Tablets by Reverse Phase High Performance Liquid Chromatography
MP Yeole, Alpana Asnani
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Abstract
A simple, sensitive and accurate RP-HPLC method has been developed and subsequently validated for simultaneous determination of telmisartan and amlodipine besylate. The separation was carried out by using mobile phase consisting of acetonitrile, methanol and phosphate buffer pH4.5 in the ratio (15:35:50) v/v and adjusted pH±0.1 with phosphoric acid. The column used was SS Wakosil-II C-18 with flow rate of 1ml/min and UV detection at 255nm. The described method was linear over a concentration range of 10.0–400μg/ml and 1–80μg/ml for the assay of telmisartan and amlodipine besylate respectively. The mean percentage recoveries obtained for telmisartan was 98.2% and for amlodipine besylate 99.5% when determined at five different levels. The proposed method is precise, accurate, selective and rapid for the simultaneous determination of telmisartan and amlodipine besylate-