2002•上海第二医科大学学报：英文版Requires access
EFFECTS AND MECHANISMS OF EMODIN OF PANCREA-TIC REGENERATION IN ACUTE PANCREATITIS IN RATS
袁耀宗, 楼恺娴, 涂水平, 翟祖康, 徐家裕, 龚自华
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Abstract
Objective To investigate the effects and mechanisms of emodin (extraction from Chinese herb Rhubard ) on pancreatic repair and regeneration in caerulein-induced pancreatitis in rats. Methods Six-ty rats were randomly divided into three groups : Group Ⅰ( control group), Group Ⅱ ( non-treated group),Group Ⅲ(emodin treated group). Acute pancreatitis was induced by intra-peritoneal infusion of caerulein in rats, emodin was administered intravenously at the time of induction of pancreatitis and 24, 48 and 72h after-wards. Rats were sacrificed at 6,24,48,72 and 96h after induction of pancreatitis. The expression of TGFβ1 and EGF mRNA were evaluated by reverse transcription-polymerase chain reaction (RT-PCR); pancreatic tissue DNA synthesis was measured by ^3H-thymidine incorporation method in vitro ; and total protein content was as-sessed by Lowry' s method. Results The serum amylase level decreased significantly in Group Ⅲ in compar-ison with Group Ⅱ . Expressions of TGFβ1 mRNA and EGF mRNA were undetectable in normal pancreas and in the pancreas of Group Ⅱ at 6h but could be observed from 24h up to 96h after the induction of pancreatitis.TGFβ1 mRNA could be detected at 6h after treatment with emodin , and it was increased signifwantly in Group Ⅲ as compared with Group t7 at 24 and 48h. EGF mRNA increased significantly in Group Ⅲ as compared with Group Ⅱ at 48h. Pancreatic tissue DNA synthesis showed a significant decrease at 72h following the induction of pancreatitis, and a marked increase was observed at 96h after treatment with emodin. Within 48h of the in-duction of pancreatitis , total protein content in pancreatic tissue declined, and there was a remarkable increasein Group Ⅲ at 96h. Conclusion Effects of emodin on pancreatic tissue regeneration of acute pancreatitis in rats might be attributed to the enhancement of TGFβ1 and EGF expression which subsequently increases total protein content and DNA synthesis, thus accelerates pancreatic repair and regeneration.