Analysis of genetic relatedness of genetic resources of Tagetes as revealed by ISSR.

【Objective】 ISSR markers were used to study the genetic relationship and investigate the genetic diversity and genetic structure for providing a theoretical basis and technical support for appropriate conservation and application of existing genetic resources of Tagetes. 【Method】 Genetic relationship of 29 accessions of Tagetes erecta and 2 accessions of T. patula were analyzed using ISSR markers. 【Result】 The genomic DNA of 31 accessions of genus Tagetes was amplified with 11 primers,the bands produced by each primer ranged from 5 to 11,and 6.8 bands were obtained on average,the polymorphic loci ranged from 40%-100%. The cluster analysis of ISSR data indicated that the resources classified by ISSR was basically in accordance with that by traditional method,the accessions were generally classified into 2 groups of different species,and cultivars of same lines were clustered into a subgroup. 【Conclusion】 The genetic relationship and genetic diversity of genus Tagetes could be assessed efficiently by ISSR markers.