Genetic variations of Hinf I, Alu I and Pst I loci within POU1F1 gene exon 6 and its association with growth traits in Jiaxian Red cattle.

[Objective] The objective of the present study was to study the genetic effects of polymorphisms of HinfⅠ,AluⅠ and PstⅠ loci within POU1F1 gene exon 6 on Jianxian Red cattle growth traits. [Method] POU1F1 gene exon 6 polymorphic fragments were detected by PCR-RFLP technique using 144 Jianxian Red cattle.Association analysis of POU1F1 gene exon 6 polymorphism with growth traits was also conducted.[Result] The results showed that 451 bp PCR products digested with HinfⅠ demonstrated polymorphisms.Moreover,the frequencies of allele AA,AB and BB genotype of Jiaxian red cattle were 0.187 5,0.305 6 and 0.506 9,accordingly,the frequencies of allele A and B were 0.340 3 and 0.659 7 respectively.The homozygositiy,heterozytosiy,the effective allele numbers,and PIC value of POU1F1 gene exon 6 HinfⅠ locus were 0.551 0,0.449 0,1.814 8 and 0.348 2 respectively.As this locus belonged to the middle polymorphic index,it was suggested to be an effective evaluation parameter for studying genetic resource. However,no polymorphism was found at POU1F1 gene exon 6 AluⅠ and Pst I loci.The correlation of HinfⅠ polymorphisms of POU1F1 gene exon 6 and growth traits was analyzed and the result showed that there was significant relationship between Hinf I polymorphism and body length (P0.05).The individual with genotype BB had longer body length than the individual with genotype AB and AA,suggesting the allele B had significant effect on body length of Jianxian red cattle. [Conclusion] The genotype BB was significantly correlated with the fine performance and the selection of the allele B was better for the improvement.