Effects of applying biogas fertilizer on nitrogen-fixation bacteria in greenhouse soil.

The paper studied the effects of the combination of biogas fertilizer,pig manure and chemical fertilizer on nitrogen-fixation bacteria in greenhouse soil.In the experiment,L402 tomato is chosen as sample,split-plot design is adopted and pig manure,biogas sludge are utilized as base fertilizers in which the organic matter contents are equivalent,chemical fertilizer and biogas slurry are used as top fertilizers in which the N,P and K contents are equivalent.The results are as followings:along with growth progress,the amount of nitrogen-fixation bacteria in the soil is becoming more and more in each fertilization treatment.Applying biogas sludge as base fertilizer is more favorable to promote nitrogen-fixation bacteria growth than that of applying pig manure.From tomato seedling to ripening period,the amount of nitrogen-fixation bacteria is much more with chemical fertilizer as top fertilizer than that with biogas slurry as top fertilizer.Applying pig manure and chemical fertilizer、biogas sludge and biogas slurry have better effects in increasing the amount of nitrogen-fixation bacteria than those of applying pig manure and biogas slurry、biogas sludge and chemical fertilizer,respectively.