ScholarLens Back to search
2015•Unpublished venueOpen access

The Effect and Mechanism of Apoptosis on Hela Cells induced by Artesunate

Guoquan Wang, Chao Xu, Sha Li, Yan Jin, Zongmiao Hu, Chenguang Liang, Linzhong Yu, Enhu Zhang

Open full text 0 citations

Abstract

Cytotoxic activity of Artesunate(ART) was determined by MTT assay at various concentrations ranging from 0 to 100 μmol/L on Hela cells.The apoptosis effect of Hela cells treated with ART was observed by a phase contrast microscope.The Apoptosis and its mechanism induced by ART were also studied by real-time PCR (RT-PCR) and Western blot.The results showed that ART has significant proliferation inhibition effect to Hela cells and the IC 50 value is 35.4μmol/L for 24 h.The expression of Bcl-2 decreased and the expression of Caspas-9 and Bid increased in the Hela cells treated with ART at concentrations from 0 to 100 μmol/L.These data suggest that the apoptotic effect of ART is mediated through mitochondrial pathway in Hela cells.

Open-access reader

About this research paper

What this paper is about

Cytotoxic activity of Artesunate(ART) was determined by MTT assay at various concentrations ranging from 0 to 100 μmol/L on Hela cells.The apoptosis effect of Hela cells treated with ART was observed by a phase contrast microscope.The Apoptosis and its mechanism induced by ART were also studied by real-time PCR (RT-PCR) and Western blot.The results showed that ART has significant proliferation inhibition effect to Hela cells and the IC 50 value is 35.4μmol/L for 24 h.The expression of Bcl-2 decreased and the expression of Caspas-9 and Bid increased in the Hela cells treated with ART at concentrations from 0 to 100 μmol/L.These data suggest that the apoptotic effect of ART is mediated through mitochondrial pathway in Hela cells.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Cytotoxic activity of Artesunate(ART) was determined by MTT assay at various concentrations ranging from 0 to 100 μmol/L on Hela cells.The apoptosis effect of Hela cells treated with ART was observed by a phase contrast microscope.The Apoptosis and its mechanism induced by ART were also studied by real-time PCR (RT-PCR) and Western blot.The results showed that ART has significant proliferation inhibition effect to Hela cells and the IC 50 value is 35.4μmol/L for 24 h.The expression of Bcl-2 decreased and the expression of Caspas-9 and Bid increased in the Hela cells treated with ART at concentrations from 0 to 100 μmol/L.These data suggest that the apoptotic effect of ART is mediated through mitochondrial pathway in Hela cells.

Key concepts: HeLa, Apoptosis, Western blot, Molecular biology, Chemistry, MTT assay, IC50, Artesunate

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
The Effect and Mechanism of Apoptosis on Hela Cells induced by Artesunate — Research Paper | ScholarLens