2011•Unpublished venueRequires access
Development and Validation of Spectrophotometric method for determination of Emtricitabine and Tenofovir Disoproxil Fumarate in Bulk and Tablet dosage form
Anindita Behera, Aurobinda Parida, Amit Kumar Meher, Dannana Gowri, Swapan Kumar Moitra, Sudam Chandra
Open publisher page 13 citations
Abstract
Three simple Spectrophotometric methods have been developed for simultaneous estimation of Emtricitabine and Tenofovir Disoproxil Fumarate from tablet dosage form. Method A is Least Square method, involves the measurement of Emtricitabine and Tenofovir Disoproxil Fumarate at their λmax at 281.0 nm and 260.5nm respectively. Method B is First order derivative spectroscopy, wavelength selected for quantitation were 234.5nm for Emtricitabine (zero cross for Tenofovir Disoproxil Fumarate ) and 281.0nm for Tenofovir Disoproxil Fumarate (zero cross for Emtricitabine). Method C is Area under Curve method, AUC in the range of 278.0- 283.0nm (for Emtricitabine) and 258.0- 262.0nm (for Tenofovir Disoproxil Fumarate) were selected for the analysis. The linearity lies between 5-25μg/ml and 10-50μg/ml for Emtricitabine and Tenofovir Disoproxil Fumarate respectively for method A, B and C. The accuracy and precision of the methods were determined and validated statically. All the methods showed good reproducibility and recovery with low % RSD.