THE EFFECTS OF SHORT AND MIDDLE TIMES AEROBIC EXERCISE WITH HIGH INTENSITIES ON INGREDIENTS ANTIOXIDANT IN FEMALE SPRAGUE DAWLEY RATS

Introduction: Different physical activities have an effects on antioxidant system. Variety, period and intensity of activities are necessary in improvement of safety in society. The purpose of this study was to evaluate the effect of high intensity training with consumption of coenzyme Q10 (CoQ10) on antioxidant enzymes activities in female Sprague Dawley rats. Methods: A total of 50 female Sprague dawley rats were divided into 4 groups: control, CoQ10, CoQ10 + exercise and exercise. Some groups were supplemented with CoQ10 (20 mg/kg) and the others were training with exercise programs for 4 weeks. The exercise programs were performed 5 times in a week with speed (10-17 m/s) in slope range (5< slope < 15) for 15-60 minutes. After that, Blood samples were drawn from heart and Enzyme activities of SOD and CAT were measured just before, after training and 24 hours later. The activities of the enzymes were determined by using enzyme linked immune sorbent assay kit (ELISA). Data were analyzed using one-way ANOVA test. Result: The results showed that high intensity training and CoQ10 had significant effects on SOD enzyme activity in groups of rats which trained with CoQ10+ exercise or exercise. Also, average CAT enzyme activity in rats which received CoQ10, showed a significant increase compared with other groups. Conclusion: It seems that using of CoQ10 with doing regulatory aerobic exercise after training and 24 hours later can cause adaptation in antioxidant enzyme system. However, CAT activity was decreased.