Research Article Isolation of Active Constituents Derived From Whole Plant of Bauhinia Purpurea

The aim of the present investigation was to isolate the active components present in whole plant of Bauhinia purpurea. The plant were extracted with various solvents (pet. ether, ethyl acetate and methanol), ethyl acetate fraction was found to be more active among them. The preliminary phytochemical results revealed that flavonoids and amino acids as active constituents in ethyl acetate extract of Bauhinia purpurea. The ethyl acetate extract of Bauhinia purpurea was undergone column chromatography with different solvent fractions. Hence, two compounds were isolated from ethyl acetate extract of Bauhinia purpurea with the compound 1 was eluted with benzene: Chloroform 90:10 v/v and compound 2 were eluted with eluted with ethyl acetate: methanol 80:20 v/v. The structures of the two isolated compounds were characterized by using FT-IR, NMR and Mass spectrophotometric methods. Thus, the compound 1 was characterized as ethyl 2-amino-5-hydroxy3,6,6-trimethyl heptonate (C12H25NO3) and compound 2 was characterized as 3,6,7-trihydroxy-2-(3methoxyphenyl)-4H-chromen-4-one(C16H12O6).Therefore, further biological investigations need to be carried out isolated compounds present in this plant.