2011•Unpublished venueRequires access

Toona Sinensis enhanced osteosarcoma cell apoptosis via increasing Bax/Bcl-2 ratio

LC Tai, Gou‐Jen Wang

Abstract

METHODS Three osteosarcoma cell lines, SaOs-2, U2Os and MG-63, were used in this study. Normal primary human osteoblasts (hOBs) were also used in this study for comparison. Cell viability, cytotocixity, apoptosis/necrosis staining and apoptosis related protein levels were examined by using MTT assay, lactate dehydrogenase (LDH) leakage, flow cytometry and Western blot, respectively.

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Available abstract

Key concepts: Apoptosis, Viability assay, Lactate dehydrogenase, Flow cytometry, Osteosarcoma, MTT assay, Western blot, Chemistry